Mapping RNA-protein interactions using iodine footprinting.

@article{Nilsen2014MappingRI,
  title={Mapping RNA-protein interactions using iodine footprinting.},
  author={Timothy W. Nilsen},
  journal={Cold Spring Harbor protocols},
  year={2014},
  volume={2014 12},
  pages={
          1337-41
        }
}
Footprinting methods are used to determine the binding site of a protein on an RNA. They are based on the fact that a protein bound to an RNA protects the RNA from cleavage by chemicals or nucleases. The footprinting method described here relies on the ability of iodoethanol to cleave the backbone of RNA when a phosphodiester bond contains sulfur in place of a nonbridging oxygen. A potential advantage of using iodoethanol for cleavage is that one can prepare RNAs that contain selective thiol… CONTINUE READING

Citations

Publications citing this paper.

Nucleotide analog interference mapping.

  • Cold Spring Harbor protocols
  • 2015
VIEW 3 EXCERPTS
CITES METHODS
HIGHLY INFLUENCED

References

Publications referenced by this paper.
SHOWING 1-10 OF 13 REFERENCES