Mapping HA-tagged protein at the surface of living cells by atomic force microscopy.

@article{Formosa2015MappingHP,
  title={Mapping HA-tagged protein at the surface of living cells by atomic force microscopy.},
  author={C{\'e}cile Formosa and V{\'e}ronique Lachaize and C{\'e}line Gal{\'e}s and Marie-Pierre Rols and H{\'e}l{\`e}ne Martin-Yken and Debora Trichez and Rapha{\"e}l Emmanuel Duval and E Dague},
  journal={Journal of molecular recognition : JMR},
  year={2015},
  volume={28 1},
  pages={1-9}
}
Single-molecule force spectroscopy using atomic force microscopy (AFM) is more and more used to detect and map receptors, enzymes, adhesins, or any other molecules at the surface of living cells. To be specific, this technique requires antibodies or ligands covalently attached to the AFM tip that can specifically interact with the protein of interest. Unfortunately, specific antibodies are usually lacking (low affinity and specificity) or are expensive to produce (monoclonal antibodies). An… CONTINUE READING
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