Mapping 70S ribosomes in intact cells by cryoelectron tomography and pattern recognition.

@article{Ortiz2006Mapping7R,
  title={Mapping 70S ribosomes in intact cells by cryoelectron tomography and pattern recognition.},
  author={Julio O. Ortiz and Friedrich F{\"o}rster and Julia K{\"u}rner and Alexandros A. Linaroudis and Wolfgang Baumeister},
  journal={Journal of structural biology},
  year={2006},
  volume={156 2},
  pages={
          334-41
        }
}
Cryoelectron tomography (CET) combines the potential of three-dimensional (3D) imaging with a close-to-life preservation of biological samples. It allows the examination of large and stochastically variable structures, such as organelles or whole cells. At the current resolution it becomes possible to visualize large macromolecular complexes in their functional cellular environments. Pattern recognition methods can be used for a systematic interpretation of the tomograms; target molecules are… CONTINUE READING
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