Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest

@article{Ji2016MapbasedCA,
  title={Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest},
  author={Hyeonso Ji and Sung-Ryul Kim and Yul-Ho Kim and Jung Pil Suh and Hyang-Mi Park and Nese Sreenivasulu and Gopal Misra and Suk‐Man Kim and Sherry Lou Hechanova and Hakbum Kim and Gang-Seob Lee and UngHan Yoon and Taeho Kim and Hyemin Lim and Suk-Chul Suh and Jungil Yang and Gynheung An and Kshirod K. Jena},
  journal={Scientific Reports},
  year={2016},
  volume={6}
}
Brown planthopper (BPH) is a phloem sap-sucking insect pest of rice which causes severe yield loss. We cloned the BPH18 gene from the BPH-resistant introgression line derived from the wild rice species Oryza australiensis. Map-based cloning and complementation test revealed that the BPH18 encodes CC-NBS-NBS-LRR protein. BPH18 has two NBS domains, unlike the typical NBS-LRR proteins. The BPH18 promoter::GUS transgenic plants exhibited strong GUS expression in the vascular bundles of the leaf… 

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