Manual and continuous-flow colorimetry of triacylglycerols by a fully enzymic method.

  title={Manual and continuous-flow colorimetry of triacylglycerols by a fully enzymic method.},
  author={ROBERT E. Megraw and Diane E. Dunn and Homer G. Biggs},
  journal={Clinical chemistry},
  volume={25 2},
We describe a fully enzymic method for manual and continuous-flow colorimetric assay of triacylglycerols (triglycerides) in serum. Triglycerides are enzymically hydrolyzed in 10 min by lipase and microbial esterase. The resulting free glycerol is measured enzymically by glycerol kinase and glycerol-3-phosphate dehydrogenase. The NADH so formed is oxidized by coupling with a tetrazolium salt/diaphorase system. The test follows Beer's law to 8 g/L, and the final color is stable for at least 1 h… 
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