Manual and continuous-flow colorimetry of triacylglycerols by a fully enzymic method.

@article{Megraw1979ManualAC,
  title={Manual and continuous-flow colorimetry of triacylglycerols by a fully enzymic method.},
  author={ROBERT E. Megraw and Diane E. Dunn and Homer G. Biggs},
  journal={Clinical chemistry},
  year={1979},
  volume={25 2},
  pages={
          273-8
        }
}
We describe a fully enzymic method for manual and continuous-flow colorimetric assay of triacylglycerols (triglycerides) in serum. Triglycerides are enzymically hydrolyzed in 10 min by lipase and microbial esterase. The resulting free glycerol is measured enzymically by glycerol kinase and glycerol-3-phosphate dehydrogenase. The NADH so formed is oxidized by coupling with a tetrazolium salt/diaphorase system. The test follows Beer's law to 8 g/L, and the final color is stable for at least 1 h… 
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References

SHOWING 1-9 OF 9 REFERENCES
Quantitative determination of serum triglycerides by the use of enzymes.
TLDR
A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
Fully automated, all-enzymatic triglyceride method adapted to the GEMSAEC centrifugal analyzer, with use of an aqueous triolein standard.
TLDR
A completely automated enzymatic method for estimating serum triglycerides with a centrifugal analyzer (ENI-GEMSAEC) is described, which cornpare well with those obtained by an automated fluorometric procedure and a manualenzymatic procedure.
Triglycerides Determination after Enzymatic Hydrolysis
Publisher Summary Serum triglycerides can be specifically and quantitatively hydrolyzed by enzymes. Lipases hydrolyze triglycerides only if the substrate is present as an emulsion. The lipase
Determination of glycerides in blood serum.
  • L. Carlson, L. Wadstrom
  • Chemistry, Medicine
    Clinica chimica acta; international journal of clinical chemistry
  • 1959
TLDR
The method is discussed and compared with earlier methods for the estimation of the glycerides in blood serum and the basic principle is the determination of the Glycerol part of theglycerides after separation of the phospholipids on silicic acid.
Micromethod for the direct determination of serum triglycerides.
Abstract A microprocedure for the direct determination of triglyceride concentrations in biologic specimens is presented. The method depends on the quantitative removal of phosphatides from the
1,4-Dihydro-3,5-diacetyl lutidine. A basis for triglyceride determination in biological samples.
An experiment for the organic laboratory that demonstrates the relationship between basic chemistry and health care; the synthesis involved is an example of the Hantzsh synthesis of a heterocyclic
  • 1974
  • 1973