Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma-membrane proteins in Schizosaccharomyces pombe

@article{Nakase2010MannosylinositolPI,
  title={Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma-membrane proteins in Schizosaccharomyces pombe},
  author={Mai Nakase and Motohiro Tani and Tomotake Morita and Hiroko Kitamoto and Jun Kashiwazaki and Taro Nakamura and Akira Hosomi and Naotaka Tanaka and Kaoru Takegawa},
  journal={Journal of Cell Science},
  year={2010},
  volume={123},
  pages={1578 - 1587}
}
In Saccharomyces cerevisiae, three classes of sphingolipids contain myo-inositol – inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC) and mannosyldiinositol phosphorylceramide [M(IP)2C]. No fission yeast equivalent of Ipt1p, the inositolphosphotransferase that synthesizes M(IP)2C from MIPC, has been found in the Schizosaccharomyces pombe genome. Analysis of the sphingolipid composition of wild-type cells confirmed that MIPC is the terminal and most abundant complex… Expand
Expression of budding yeast IPT1 produces mannosyldiinositol phosphorylceramide in fission yeast and inhibits cell growth.
In Saccharomyces (Sacc.) cerevisiae, the final step of the complex sphingolipid biosynthetic pathway requires Ipt1p for synthesis of mannosyldiinositol phosphorylceramide [M(IP)(2)C]. No fissionExpand
Synthesis of mannosylinositol phosphorylceramides is involved in maintenance of cell integrity of yeast Saccharomyces cerevisiae
TLDR
It is found that a specific group of glycosyltransferases involved in the synthesis of mannan‐type N‐glycans is essential for the growth of cells lacking MIPC synthases (Sur1 and Csh1). Expand
Mannosylinositol phosphorylceramides and ergosterol coodinately maintain cell wall integrity in the yeast Saccharomyces cerevisiae
TLDR
It is suggested that MIPC and ergosterol are coordinately involved in maintenance of CWI, and the activation of Slt2 suppressed the CWI defect caused by these metabolic defects. Expand
Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid permease Aat1p.
TLDR
Results suggest that Pub1p-mediated ubiquitination is required for retention of Aat1 at the Golgi under nitrogen-sufficient conditions, and indicate that ubiquitinations is an important determinant for localization and regulation of the Aat 1p permease in S. pombe. Expand
Functional analysis of putative phosphoenolpyruvate transporters localized to the Golgi apparatus in Schizosaccharomyces pombe.
TLDR
Analysis of putative phosphoenolpyruvate (PEP) transporter genes in S. pombe revealed that Pet1p and Pet2p are likely involved in transporting PEP from the cytoplasm into the Golgi. Expand
Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis.
TLDR
GntA was shown to use UDP-N-acetylglucosamine as donor substrate to generate a glycolipid product resistant to saponification and to digestion by phosphatidylinositol-phospholipase C as expected for GlcNAcα1,2IPC. Expand
Inositol lipid synthesis is widespread in host-associated Bacteroidetes
TLDR
Inositol sphingolipid production, via one of the two pathways, is widespread in host-associated Bacteroidetes, and may be implicated in host interactions both indirectly via the capsule and directly through inositol lipid provisioning. Expand
Involvement of complex sphingolipids and phosphatidylserine in endosomal trafficking in yeast Saccharomyces cerevisiae
TLDR
Results suggested that specific complex sphingolipids and phosphatidylserine are co‐ordinately involved in specific vesicular trafficking pathway. Expand
The Ubiquitin Ligase Ubr11 Is Essential for Oligopeptide Utilization in the Fission Yeast Schizosaccharomyces pombe
TLDR
The role of potential peptide transporters in the yeast Schizosaccharomyces pombe was investigated and the peptidomimetic proteasome inhibitor MG132 was still operative in a strain lacking all known PTR and OPT peptide Transporters. Expand
Vsl1p cooperates with Fsv1p for vacuolar protein transport and homotypic fusion in Schizosaccharomyces pombe.
TLDR
It is suggested that Vsl1p and Fsv1p are required for vacuolar protein transport and membrane fusion, and they function cooperatively with Pep12p in the same membrane-trafficking step. Expand
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TLDR
It is shown that a homolog (open reading frameYDR072c), termed Ipt1 (inositolphosphotransferase1) is necessary for synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant and complex sphingolipid in S. cerevisiae. Expand
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TLDR
It is shown that open reading frame YBR161w, renamed CSH1, is functionally homologous to CSG1 and that deletion of both genes abolishes MIPC and M(IP)2C synthesis without affecting protein mannosylation, indicating that protein sorting in the late Golgi of yeast does not require production of mannosyldi-IPC. Expand
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TLDR
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TLDR
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TLDR
The results predict the existence of an enzyme(s) or process(es) essential for the coordination of S. pombe cell wall formation and division that is, in turn, regulated by a sphingolipid metabolite. Expand
SUR1 (CSG1 / BCL21), a gene necessary for growth of Saccharomyces cerevisiae in the presence of high Ca2+ concentrations at 37° C, is required for mannosylation of inositolphosphorylceramide
TLDR
It is demonstrated that SUR1/CSG1 is both genetically and biochemically related to CSG2, a gene encoding an intracellular Cu2+ transporter required for IPC mannosylation. Expand
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