Mannose dependent tightening of the rat ependymal cell barrier. In vivo and in vitro study using neoglycoproteins.

@article{Kuchler1994MannoseDT,
  title={Mannose dependent tightening of the rat ependymal cell barrier. In vivo and in vitro study using neoglycoproteins.},
  author={Sabine Kuchler and Michael Graff and Serge Gobaille and Guy Vincendon and A. C. Roche and Jean Pierre Delaunoy and M. Monsigny and J. P. Zanetta},
  journal={Neurochemistry international},
  year={1994},
  volume={24 1},
  pages={43-55}
}
The possible role of carbohydrate binding proteins (lectins) and glycoconjugates in the formation of junctions ensuring tightening between ependymal cells was studied using synthetic glycoconjugates, the neoglycoproteins. These compounds are prepared by substituting bovine serum albumin with sugar residues and additional labelling (or not) with fluorescein or biotin. Injections of these components into the cerebral ventricles of adult rats resulted in a binding pattern which could be related to… CONTINUE READING

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