Corpus ID: 24864248

Mannose, glucosamine and inositol monophosphate inhibit the effects of insulin on lipogenesis. Further evidence for a role for inositol phosphate-oligosaccharides in insulin action.

  title={Mannose, glucosamine and inositol monophosphate inhibit the effects of insulin on lipogenesis. Further evidence for a role for inositol phosphate-oligosaccharides in insulin action.},
  author={Fausto Machicao and Joanne Mushack and E. Seffer and Brian F. Ermel and H. U. H{\"a}ring},
  journal={The Biochemical journal},
  volume={266 3},
The mechanism of insulin signalling is not yet understood in detail. Recently, a role for inositol phosphate (IP)-oligosaccharides as second messengers transmitting the insulin signal at the post-kinase level was proposed. To evaluate this hypothesis further, we studied whether IP-oligosaccharides isolated from 'haemodialysate' have insulin-like activity. We found that these compounds mimic, in a dose-dependent fashion, the following effects of insulin in adipocytes. (1) Lipogenesis… Expand
Insulin-like effects of inositol phosphate-glycan on messenger RNA expression in rat hepatocytes.
Data indicate that both positive and negative effects of insulin on the regulation of gene expression are mimicked by IPG, and the effect of IPG was dose dependent and could not be reproduced by galactose, glucosamine, or myo-inositol. Expand
Changes in the insulin-sensitive glycosyl-phosphatidyl-inositol signalling system with aging in rat hepatocytes.
The hypothesis that insulin resistance associated with aging is accompanied by an impairment of the glycosyl-PtdIns-dependent cellular signalling system is supported. Expand
Phosphoinositolglycan-Peptides from Yeast Potently Induce Metabolic Insulin Actions in Isolated Rat Adipocytes, Cardiomyocytes, and Diaphragms.
The structure of the resulting PIG-P, NH2-Tyr-Cys-Asn-ethanolamine-PO4-6, was revealed by amino acid analysis and Dionex exchange chromatography of fragments generated enzymatically or chemically from the neutral glycan core a... Expand
Lack of effects of myo-inositol on metabolism of primary rat adipocytes
Abstract Myo-inositol is a ubiquitous cyclitol, has an important regulatory role, and its intracellular depletion is associated with pathological changes. Effects of myo-inositol on adipose tissueExpand
Membrane association of lipoprotein lipase and a cAMP-binding ectoprotein in rat adipocytes.
Data suggest that in rat adipocytes GPI-proteins are not readily released from the cell surface upon lipolytic cleavage, but remain associated through a receptor which specifically recognizes the terminal inositol (cyclic) monophosphate epitope of the (G)PI-PLC-cleaved GPI moiety. Expand
Inositol-phosphoglycan inhibits calcium oscillations in hepatocytes by reducing calcium entry.
It is concluded that IPG inhibits the hepatocyte Ca 2+ oscillatory by reducing the continuous Ca2+ influx that is required to sustain oscillations in [Ca2+]i by almost completely abolished the Mn2+ quench rate. Expand
Potential role and therapeutic interests of myo-inositol in metabolic diseases.
The aim of this review is to focus on the potential benefits of a dietary supplement of myo-inositol, by far the most common inositol isomer in foodstuffs, in human disorders associated with insulin resistance, in prevention or treatment of some diabetic complications (neuropathy, nephropathy, cataract), and the relevance of such a nutritional strategy for each context on the basis of the clinical and/or animal studies. Expand
TPA inhibits insulin stimulated PIP hydrolysis in fat cell membranes: evidence for modulation of insulin dependent phospholipase C by proteinkinase C.
Fat cell membranes contain an insulin dependent phospholipase C which is inhibited by TPA most likely via serine phosphorylation through proteinkinase C. Expand
Insulin second messengers
  • P. Strålfors
  • Biology, Medicine
  • BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1997
The phospho‐oligosaccharide appears to mediate many metabolic effects of insulin through control of the phosphorylation state of key regulatory metabolic enzymes, and diacylglycerol may mediate insulin's stimulation of glucose transport over the plasma membrane. Expand
Isolation of phosphooligosaccharide/phosphoinositol glycan from caveolae and cytosol of insulin-stimulated cells
It is demonstrated that [32]phosphate in freshly isolated adipocytes and [3H]galactose in cultured hepatoma cells (H4IIE) labeled the same set of three different glycolipids, with the radiolabel made water soluble by phosphatidylinositol(glycan)- specific phospholipase C or D catalyzed hydrolysis. Expand