Manipulation of thiocillin variants by prepeptide gene replacement: structure, conformation, and activity of heterocycle substitution mutants.

@article{Bowers2010ManipulationOT,
  title={Manipulation of thiocillin variants by prepeptide gene replacement: structure, conformation, and activity of heterocycle substitution mutants.},
  author={Albert A Bowers and Michael G Acker and Alexander Koglin and Christopher T Walsh},
  journal={Journal of the American Chemical Society},
  year={2010},
  volume={132 21},
  pages={7519-27}
}
Bacillus cereus ATCC 14579 converts the C-terminal 14 residues of a 52-mer prepeptide into a related set of eight variants of the thiocillin subclass of thiazolyl peptide antibiotics by a cascade of post-translational modifications that alter 13 of those 14 residues. We have introduced prepeptide gene variants into a knockout strain to conduct an alanine scan of all 14 progenitor residues, as well as a serine scan of the six cysteine residues that are converted to thiazoles in the mature… CONTINUE READING