Manipulation of the napin primary structure alters its packaging and deposition in transgenic tobacco (Nicotiana tabacum L.) seeds

@article{Scarafoni2004ManipulationOT,
  title={Manipulation of the napin primary structure alters its packaging and deposition in transgenic tobacco (Nicotiana tabacum L.) seeds},
  author={A. Scarafoni and R. Carzaniga and N. Harris and R. Croy},
  journal={Plant Molecular Biology},
  year={2004},
  volume={46},
  pages={727-739}
}
Napin is a 2S storage protein found in the seeds of oilseed rape (Brassica napus L.) and related species. Using protein structural prediction programs we have identified a region in the napin protein sequence which forms a `hydrophilic loop' composed of amino acid residues located at the protein surface. Targeting this region, we have constructed two napin chimeric genes containing the coding sequence for the peptide hormone leucine-enkephalin as a topological marker. One version has a single… Expand
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Isolation of a napin‐like polypeptide with potent translation‐inhibitory activity from chinese cabbage (Brassica parachinensis cv green‐stalked) seeds
  • P. Ngai, T. Ng
  • Biology, Medicine
  • Journal of peptide science : an official publication of the European Peptide Society
  • 2003
TLDR
The N‐terminal sequence of the 5 kDa subunit of the polypeptide showed high homology with other 2S storage proteins like napins and albumins and was relatively stable in the pH range 6–11 and in the temperature range 10–50°C. Expand
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In tobacco, the napA promoter directed GUS activity early in the endosperm before any visible activity could be seen in the heart-shaped embryo, and during the transition from heart to torpedo stages, the main expression of GUS was localized to the embryo. Expand
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