Manipulation of salicylate content in Arabidopsis thaliana by the expression of an engineered bacterial salicylate synthase.

@article{Mauch2001ManipulationOS,
  title={Manipulation of salicylate content in Arabidopsis thaliana by the expression of an engineered bacterial salicylate synthase.},
  author={Felix Mauch and Brigitte Mauch-Mani and Catherine Gaille and Bj{\"o}rn Kull and Dieter Haas and Cornelia Reimmann},
  journal={The Plant journal : for cell and molecular biology},
  year={2001},
  volume={25 1},
  pages={67-77}
}
Salicylic acid (SA) plays a central role as a signalling molecule involved in plant defense against microbial attack. Genetic manipulation of SA biosynthesis may therefore help to generate plants that are more disease-resistant. By fusing the two bacterial genes pchA and pchB from Pseudomonas aeruginosa, which encode isochorismate synthase and isochorismate pyruvate-lyase, respectively, we have engineered a novel hybrid enzyme with salicylate synthase (SAS) activity. The pchB-A fusion was… CONTINUE READING

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