Manipulation of mammalian cells using a single-fiber optical microbeam.

  title={Manipulation of mammalian cells using a single-fiber optical microbeam.},
  author={Samarendra Kumar Mohanty and Khyati S Mohanty and Michael W. Berns},
  journal={Journal of biomedical optics},
  volume={13 5},
The short working distance of microscope objectives has severely restricted the application of optical micromanipulation techniques at larger depths. We show the first use of fiber-optic tweezers toward controlled guidance of neuronal growth cones and stretching of neurons. Further, by mode locking, the fiber-optic tweezers beam was converted to fiber-optic scissors, enabling dissection of neuronal processes and thus allowing study of the subsequent response of neurons to localized injury. At… CONTINUE READING


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