Manipulation of Endogenous Kinase Activity in Living Cells Using Photoswitchable Inhibitory Peptides

@inproceedings{Yi2014ManipulationOE,
  title={Manipulation of Endogenous Kinase Activity in Living
Cells Using Photoswitchable Inhibitory Peptides},
  author={Jason J. Yi and Hui Wang and Marco Vilela and Gaudenz Danuser and Klaus M Hahn},
  booktitle={ACS synthetic biology},
  year={2014}
}
Optogenetic control of endogenous signaling can be an important tool for probing cell behavior. Using the photoresponse of the LOV2 domain of Avena sativa phototropin 1, we developed analogues of kinase inhibitors whose activity is light dependent. Inhibitory peptides were appended to the Jα helix, where they potently inhibited kinases in the light but were sterically blocked from kinase interaction in the dark. Photoactivatable inhibitors for cyclic-AMP dependent kinase (PKA) and myosin light… CONTINUE READING
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