Manipulation of β-glucuronidase for use as a reporter in vacuolar targeting studies

  title={Manipulation of β-glucuronidase for use as a reporter in vacuolar targeting studies},
  author={Leigh B. Farrell and Roger N. Beachy},
  journal={Plant Molecular Biology},
It has been documented that when furnished with an endomembrane signal sequence for the endoplasmic reticulum, β-glucuronidase (GUS) is N-glycosylated, resulting in the nearly complete loss of enzymatic activity. To enable use of β-glucuronidase as a reporter protein in secretory and vacuolar targeting studies, one of the two putative N-linked glycosylation sites within the GUS gene was altered by site-directed mutagenesis. The second N-linked glycosylation site was not altered because sequence… CONTINUE READING

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