Manipulation of β-glucuronidase for use as a reporter in vacuolar targeting studies

@article{Farrell1990ManipulationO,
  title={Manipulation of β-glucuronidase for use as a reporter in vacuolar targeting studies},
  author={Leigh B. Farrell and Roger N. Beachy},
  journal={Plant Molecular Biology},
  year={1990},
  volume={15},
  pages={821-825}
}
It has been documented that when furnished with an endomembrane signal sequence for the endoplasmic reticulum, β-glucuronidase (GUS) is N-glycosylated, resulting in the nearly complete loss of enzymatic activity. To enable use of β-glucuronidase as a reporter protein in secretory and vacuolar targeting studies, one of the two putative N-linked glycosylation sites within the GUS gene was altered by site-directed mutagenesis. The second N-linked glycosylation site was not altered because sequence… CONTINUE READING

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Molecular Cloning

  • J Sambrook, EF Fritsch, T Maniatis
  • A laboratory manual. Cold Spring Harbor…
  • 1989
Highly Influential
3 Excerpts

A rapid and sensitive method for the quantities of protein utilizing the principle of protein-dye binding

  • M Bradford
  • Anal Biochem 72:248-254
  • 1976
1 Excerpt

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