Manipulating substrate and pH in zymography protocols selectively distinguishes cathepsins K, L, S, and V activity in cells and tissues.

@article{Wilder2011ManipulatingSA,
  title={Manipulating substrate and pH in zymography protocols selectively distinguishes cathepsins K, L, S, and V activity in cells and tissues.},
  author={Catera L. Wilder and Keon-Young Park and Philip M. Keegan and Manu O. Platt},
  journal={Archives of biochemistry and biophysics},
  year={2011},
  volume={516 1},
  pages={52-7}
}
Cathepsins K, L, S, and V are cysteine proteases that have been implicated in tissue-destructive diseases such as atherosclerosis, tumor metastasis, and osteoporosis. Among these four cathepsins are the most powerful human collagenases and elastases, and they share 60% sequence homology. Proper quantification of mature, active cathepsins has been confounded by inhibitor and reporter substrate cross-reactivity, but is necessary to develop properly dosed therapeutic applications. Here, we detail… CONTINUE READING