Manifold sequencing: efficient processing of large sets of sequencing reactions.

@article{Lagerkvist1994ManifoldSE,
  title={Manifold sequencing: efficient processing of large sets of sequencing reactions.},
  author={A Lagerkvist and Jeneen Stewart and Maria Lagerstr{\"o}m-Ferm{\'e}r and Ulf Landegren},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 6},
  pages={2245-9}
}
Automated instruments for DNA sequencing greatly simplify data collection in the Sanger sequencing procedure. By contrast, the so-called front-end problems of preparing sequencing templates, performing sequencing reactions, and loading these on the instruments remain major obstacles to extensive sequencing projects. We describe here the use of a manifold support to prepare and perform sequencing reactions on large sets of templates in parallel, as well as to load the reaction products on a… CONTINUE READING

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