Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae.

Abstract

Neisseria gonorrhoeae has evolved a complex and novel network of oxidative stress responses, including defence mechanisms that are dependent on manganese (Mn). We performed systematic analyses at the transcriptomic and proteomic (1D SDS-PAGE and Isotope-Coded Affinity Tag [ICAT]) levels to investigate the global expression changes that take place in a high Mn environment, which results in a Mn-dependent oxidative stress resistance phenotype. These studies revealed that there were proteins regulated at the post-transcriptional level under conditions of increased Mn concentration, including proteins involved in virulence (e.g., pilin, a key adhesin), oxidative stress defence (e.g., superoxide dismutase), cellular metabolism, protein synthesis, RNA processing and cell division. Mn regulation of inorganic pyrophosphatase (Ppa) indicated the potential involvement of phosphate metabolism in the Mn-dependent oxidative stress defence. A detailed analysis of the role of Ppa and polyphosphate kinase (Ppk) in the gonococcal oxidative stress response revealed that ppk and ppa mutant strains showed increased resistance to oxidative stress. Investigation of these mutants grown with high Mn suggests that phosphate and pyrophosphate are involved in Mn-dependent oxidative stress resistance.

DOI: 10.1016/j.jprot.2009.12.001

Cite this paper

@article{Wu2010ManganeseRO, title={Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae.}, author={Hsing-Ju Wu and Kate L Seib and Yogitha N. Srikhanta and Jennifer Lobo Edwards and Stephen P. Kidd and Tina L. Maguire and Amanda J Hamilton and Kuan-Tin Pan and He-Hsuan Hsiao and Chen-Wen Yao and Sean M. Grimmond and Michael A. Apicella and Alastair G . McEwan and Andrew H. J. Wang and Michael P Jennings}, journal={Journal of proteomics}, year={2010}, volume={73 5}, pages={899-916} }