Manganese Peroxidases: Molecular Diversity, Heterologous Expression, and Applications

@inproceedings{Saroj2013ManganesePM,
  title={Manganese Peroxidases: Molecular Diversity, Heterologous Expression, and Applications},
  author={Samta Saroj and Pragati Agarwal and Swati Dubey and Rajesh P. Singh},
  year={2013}
}
Manganese peroxidases (MnPs) are a fascinating group of biocatalysts with various ecological and biotechnological implications. They are involved in the biodegradation of lignocellulose and lignin and participate in the bioconversion of other diverse recalcitrant compounds, like polycyclic aromatic hydrocarbons, chlorophenols, industrial effluents (mostly from the paper and pulp), and textile and petrochemical industries, and bioremediation of contaminated soils. This chapter presents an… 
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References

SHOWING 1-10 OF 132 REFERENCES
The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.
We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose
Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review
TLDR
The applications of ligninolytic enzymes of basidiomycetes within different industrial and biotechnological area are reviewed.
Studies on the Production of Fungal Peroxidases inAspergillus niger
TLDR
The recombinant protein showed specific activity and a spectrum profile similar to those of the native enzyme, was correctly processed at its N terminus, and had a slightly lower mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Structure and Action Mechanism of Ligninolytic Enzymes
  • D. Wong
  • Biology, Chemistry
    Applied biochemistry and biotechnology
  • 2009
TLDR
This review covers the chemical nature of lignin substrates and focuses on the biochemical properties, molecular structures, reaction mechanisms, and related structures/functions of these enzymes.
Heterologous Expression of Manganese Peroxidase in Aspergillus niger and Its Effect on Phenanthrene Removal from Soil
A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene
Recent developments in biodegradation of industrial pollutants by white rot fungi and their enzyme system
TLDR
This review has tried to cover the latest developments on enzyme systems of WRF, their low molecular mass mediators and their potential use for bioremediation of industrial pollutants.
Generation of a transformant showing higher manganese peroxidase (Mnp) activity by overexpression of Mnp gene in Trametes versicolor.
TLDR
A cDNA clone of a putative MnP-coding gene was cloned and transferred into an expression vector carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2.
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