Manganese Peroxidases: Molecular Diversity, Heterologous Expression, and Applications

  title={Manganese Peroxidases: Molecular Diversity, Heterologous Expression, and Applications},
  author={Samta Saroj and Pragati Agarwal and Swati Dubey and Rajesh P. Singh},
Manganese peroxidases (MnPs) are a fascinating group of biocatalysts with various ecological and biotechnological implications. They are involved in the biodegradation of lignocellulose and lignin and participate in the bioconversion of other diverse recalcitrant compounds, like polycyclic aromatic hydrocarbons, chlorophenols, industrial effluents (mostly from the paper and pulp), and textile and petrochemical industries, and bioremediation of contaminated soils. This chapter presents an… 
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    Archives of biochemistry and biophysics
  • 2022
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The results showed that two forms of recombinant MnP could be expressed in E. coli by varying the culture conditions during protein expression, and demonstrated that refolded MnP was a good monomer in solution, while soluble MnP predominantly existed in the oligomeric status.
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    Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  • 2017
Ligninolytic bacteria are considered a major source of ligninolytic enzymes, which are widely used in a variety of industrial and biotechnological processes. The present study aimed to isolate
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Biodegradation of winery biomass wastes by developing a symbiotic multi-fungal consortium
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The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.
We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose
Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review
The applications of ligninolytic enzymes of basidiomycetes within different industrial and biotechnological area are reviewed.
Studies on the Production of Fungal Peroxidases inAspergillus niger
The recombinant protein showed specific activity and a spectrum profile similar to those of the native enzyme, was correctly processed at its N terminus, and had a slightly lower mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Structure and Action Mechanism of Ligninolytic Enzymes
  • D. Wong
  • Biology, Chemistry
    Applied biochemistry and biotechnology
  • 2009
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Generation of a transformant showing higher manganese peroxidase (Mnp) activity by overexpression of Mnp gene in Trametes versicolor.
A cDNA clone of a putative MnP-coding gene was cloned and transferred into an expression vector carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2.