Management of multipartite genomes: the Vibrio cholerae model.

@article{Val2014ManagementOM,
  title={Management of multipartite genomes: the Vibrio cholerae model.},
  author={Marie-Eve Val and Alfonso Soler-Bistu{\'e} and Michael Jason Bland and Didier Mazel},
  journal={Current opinion in microbiology},
  year={2014},
  volume={22},
  pages={
          120-6
        }
}

Establishing a System for Testing Replication Inhibition of the Vibrio cholerae Secondary Chromosome in Escherichia coli

An E. coli strain in which chromosomal replication is driven by the replication origin of V. cholerae Chr2 is characterized, and it is found that this strain was not inhibited by vibrepin, previously found to inhibit ori2-based replication.

A checkpoint control orchestrates the replication of the two chromosomes of Vibrio cholerae

This study reveals a new checkpoint control mechanism in bacteria, and highlights possible functional interactions mediated by contacts between two chromosomes, an unprecedented observation in bacteria.

Cell division licensing in the multi-chromosomal Vibrio cholerae bacterium

It is shown that Chr2 harbours binding motifs for an inhibitor of Z-ring formation, which helps accurately position the V. cholerae divisome at mid-cell and postpones its assembly to the very end of the cell cycle.

The Divided Bacterial Genome: Structure, Function, and Evolution

A meta-analysis of completed bacterial genomes from 1,708 species is provided as a way of reviewing the abundant information present in these genome sequences, and an encompassing model to explain the evolution and function of the multipartite genome structure is provided.

Exception to the Rule: Genomic Characterization of Naturally Occurring Unusual Vibrio cholerae Strains with a Single Chromosome

The genomic characterization of strains that are an exception to the two chromosome rules: naturally occurring single-chromosome V. cholerae and NSCV2 are described, finding that the two origins of replication and associated genes are generally intact with synonymous mutations in some genes.

Genomic Plasticity of Vibrio cholerae.

  • J. EscuderoD. Mazel
  • Biology
    International microbiology : the official journal of the Spanish Society for Microbiology
  • 2017
This review will focus on two peculiar genomic features that enhance genetic plasticity in this bacterium: the division of its genome in two different chromosomes and the presence of the superintegron, a gene capture device that acts as a large, low-cost memory of adaptive functions, allowing V. cholerae to adapt rapidly.

Synchronous termination of replication of the two chromosomes is an evolutionary selected feature in Vibrionaceae

It is suggested that it is beneficial for bacterial cells with multiple chromosomes to synchronize their replication termination, potentially to optimize chromosome related processes as dimer resolution or segregation.

Exception to the Exception Rule: Synthetic and Naturally Occurring Single Chromosome Vibrio cholerae.

The pertinent findings on this exception to the exception rule are summarized, the potential utility of single-chromosome V. cholerae is discussed and fundamental questions on chromosome biology in general and DNA replication in particular are addressed.

Functionality of Two Origins of Replication in Vibrio cholerae Strains With a Single Chromosome

It is found that in N SCV1, both origins are active whereas in NSCV2 ori2 is silenced despite the fact that it is functional in an isolated context, and whether two types of origins of replication can function simultaneously on the same chromosome or one or the other origin is silenced is tested.

The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators

These findings support the hypothesis that the second Vibrionaceae chromosome arose from an ancestral plasmid, and that RctB may have evolved additional regulatory features, and support the idea that bacteria with multipartite genomes evolved distinct systems to initiate replication of secondary chromosomes.

References

SHOWING 1-10 OF 56 REFERENCES

Genome Engineering in Vibrio cholerae: A Feasible Approach to Address Biological Issues

It is shown that Dam, RctB, and ParA2/ParB2 are strictly essential for chrII origin maintenance, and it is formally demonstrated that the formation of chromosome dimers increases exponentially with chromosome size.

FtsK-Dependent Dimer Resolution on Multiple Chromosomes in the Pathogen Vibrio cholerae

It is shown that specific DNA motifs dictate its orientation of translocation, the distribution of these motifs on chromosome I and chromosome II supporting the idea that FtsK translocation serves to bring together the resolution sites carried by a dimer at the time of cell division.

The two chromosomes of Vibrio cholerae are initiated at different time points in the cell cycle

The divided genome and delayed replication of chromosome II may reduce the metabolic burden and complexity of chromosome replication by postponing DNA synthesis to the last part of the cell cycle and reducing the need for overlapping replication cycles during rapid proliferation.

Vibrio chromosomes share common history

While elements within the chromosomes may have experienced significant genetic mobility, the backbones share a common history that allows conclusions based on multilocus sequence analysis (MLSA) for one chromosome to be applied equally to both chromosomes.

The Vibrio cholerae genome contains two unique circular chromosomes.

The most notable finding is that the V. cholerae chromosome appears to be not the single chromosome reported but two unique and separate circular megareplicons.

par genes and the pathology of chromosome loss in Vibrio cholerae

It is shown that the chromosome II parAB2 genes are essential for the segregation of chromosome II but not chromosome I, which suggests that prokaryotes, like eukaryote, may possess characteristic death pathways.

Fuse or die: how to survive the loss of Dam in Vibrio cholerae

It is reported that natural fusion of chr1 and chr2 occurred by two distinct recombination pathways: homologous recombination between repeated IS elements and site‐specific recombinations between dif sites.

Natural genomic design in Sinorhizobium meliloti: novel genomic architectures.

Based on the nucleotide sequence of the whole genome, a pathway of consecutive rearrangements leading to novel genomic architectures is designed and it is found that the relative frequency of excision of cointegrated replicons is higher at the site used for the cointegration than at other sites.
...