Mammalian p53 can function as a transcription factor in yeast.

  title={Mammalian p53 can function as a transcription factor in yeast.},
  author={E. Sch{\"a}rer and R. Iggo},
  journal={Nucleic acids research},
  volume={20 7},
p53 has previously been shown to contain a transactivation domain using GAL4 fusion proteins and to bind specifically to a 33 base pair DNA sequence in immunoprecipitation assays. We show here that mammalian p53 expressed in S. cerevisiae is able to activate transcription of a reporter gene placed under the control of a CYC1 hybrid promoter containing the 33 base pair p53-binding sequence. The activation is dependent on the orientation and number of copies of the binding site. Three p53 mutants… Expand
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