Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.

  title={Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.},
  author={Marc R Fabian and G{\'e}raldine Mathonnet and Thomas R. Sundermeier and Hansruedi Mathys and Jakob T. Zipprich and Yuri V. Svitkin and Fabiola Rivas and Martin Jinek and James A Wohlschlegel and Jennifer A. Doudna and C A Chen and A B Shyu and John R. Yates and Gregory J Hannon and W. Filipowicz and Thomas F. Duchaine and Nahum Sonenberg},
  journal={Molecular cell},
  volume={35 6},
MicroRNAs (miRNAs) inhibit mRNA expression in general by base pairing to the 3'UTR of target mRNAs and consequently inhibiting translation and/or initiating poly(A) tail deadenylation and mRNA destabilization. Here we examine the mechanism and kinetics of miRNA-mediated deadenylation in mouse Krebs-2 ascites extract. We demonstrate that miRNA-mediated mRNA deadenylation occurs subsequent to initial translational inhibition, indicating a two-step mechanism of miRNA action, which serves to… CONTINUE READING
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