Mammalian inhibitors of angiotensin converting enzyme (kininase II).

@article{Ryan1979MammalianIO,
  title={Mammalian inhibitors of angiotensin converting enzyme (kininase II).},
  author={James W. Ryan and Leroy C. Martin and Alfred Chung and G A Pena},
  journal={Advances in experimental medicine and biology},
  year={1979},
  volume={120B},
  pages={599-606}
}
Urines and sera (human, guinea pig and rat) contain low molecular weight inhibitors of angiotensin converting enzyme (ACE). The urines contain ACE, but the enzyme is scarcely measurable without prior ultrafiltration or dialysis. The activity increases strikingly through three ultrafiltration steps using a membrane with a 10,000 MW retention limit. As implied, the ultrafiltrates contain inhibitory activity and can prevent the hydrolysis of [3H]benzoyl-Gly-His-Leu by ACE from any source… CONTINUE READING