Mammalian assay for site-specific DNA damage processing using the human H-ras proto-oncogene.

@article{Arcangeli1995MammalianAF,
  title={Mammalian assay for site-specific DNA damage processing using the human H-ras proto-oncogene.},
  author={Loretta Arcangeli and Kandace J. Williams},
  journal={Nucleic acids research},
  year={1995},
  volume={23 12},
  pages={2269-76}
}
The human genomic H-ras proto-oncogene was inserted into an Epstein-Barr virus (EBV) vector (p220.2) that replicates synchronously with the cell cycle. Unique restriction enzyme sites, 30 bp apart, were created on either side of codon 12 to enable the construction of gapped heteroduplex (GHD) DNA. Depending upon experimental protocol, the gap could be located either on the coding (non-transcribed) strand or the non-coding (transcribed) strand. GHD DNA was created using a 1.8 kb segment of H-ras… CONTINUE READING