Mammalian Telomeres End in a Large Duplex Loop

@article{Griffith1999MammalianTE,
  title={Mammalian Telomeres End in a Large Duplex Loop},
  author={J. Griffith and L. Comeau and S. Rosenfield and R. M. Stansel and A. Bianchi and H. Moss and T. Lange},
  journal={Cell},
  year={1999},
  volume={97},
  pages={503-514}
}
Mammalian telomeres contain a duplex array of telomeric repeats bound to the telomeric repeat-binding factors TRF1 and TRF2. Inhibition of TRF2 results in immediate deprotection of chromosome ends, manifested by loss of the telomeric 3' overhang, activation of p53, and end-to-end chromosome fusions. Electron microscopy reported here demonstrated that TRF2 can remodel linear telomeric DNA into large duplex loops (t loops) in vitro. Electron microscopy analysis of psoralen cross-linked telomeric… Expand
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