Malignant transformation of B lymphoma cell line BJAB by Epstein–Barr virus‐encoded small RNAs

@article{Yamamoto2000MalignantTO,
  title={Malignant transformation of B lymphoma cell line BJAB by Epstein–Barr virus‐encoded small RNAs},
  author={Noriko Yamamoto and T Takizawa and Youichi Iwanaga and Norio Shimizu and Noriko Yamamoto},
  journal={FEBS Letters},
  year={2000},
  volume={484}
}
Modulation of innate immunity system by Epstein–Barr virus‐encoded non‐coding RNA and oncogenesis
TLDR
A model that highlights the functions of EBERs in EBV‐mediated oncogenesis in BL cells is presented and it is shown that recombinant EBVs carrying only the EBER2 gene play a greater role in the growth transformation of B lymphocytes than EBVscarrying only theEBER1 gene.
The role of EBERs in oncogenesis.
TLDR
The EBERs confer clonability in soft agarose, tumourigenicity in mice, and resistance to apoptosis against various stimuli in BL, and open the way toward the new concept that RNA molecules can act in oncogenesis.
Role of EBERs in the pathogenesis of EBV infection.
The labyrinth of interactions of Epstein–Barr virus‐encoded small RNAs
TLDR
The current understanding of the complex interactions of EBERs with various cellular factors and the potential pathways by which these small RNAs are able to influence EBV‐infected cells to proliferate and to induce tumorigenesis is summarized.
Stable expression of EBERs in immortalized nasopharyngeal epithelial cells confers resistance to apoptotic stress
TLDR
The results suggest that EBER expression may confer an apoptotic‐resistant phenotype in immortalized nasopharyngeal epithelial cells.
Growth-Promoting Properties of Epstein-Barr Virus EBER-1 RNA Correlate with Ribosomal Protein L22 Binding
TLDR
It is demonstrated thatBL cells expressing mutated EBER-1 RNAs rendered incapable of binding L22 have significantly reduced capacity to enhance cell growth potential relative to BL cells expressing wild-type EBERs.
Epstein–Barr virus RNA confers resistance to interferon‐α‐induced apoptosis in Burkitt's lymphoma
TLDR
Results indicate that EBV‐encoded poly(A)− RNAs confer resistance to IFN‐α‐induced apoptosis via binding to PKR and inhibition of its phosphorylation, the first report that the virus counteractsIFN‐induced suicides in virus‐associated tumors.
Role of EBER and BARF1 in nasopharyngeal carcinoma (NPC) tumorigenesis.
  • K. Takada
  • Biology, Medicine
    Seminars in cancer biology
  • 2012
Epstein-Barr Virus-Encoded RNAs: Key Molecules in Viral Pathogenesis
TLDR
The current understanding of the functions of EBERs is summarized, including the interactions with cellular factors through which Ebers contribute to EBV-mediated pathogenesis, and EBER1 was detected in the sera of patients with activeEBV-infectious diseases.
Ribosomal protein L22 inhibits regulation of cellular activities by the Epstein-Barr virus small RNA EBER-1.
TLDR
It is suggested that the association of L22 with Eber-1 in EBV-infected cells can attenuate the biological effects of the viral RNA, including both the inhibition of PKR and additional mechanism(s) by which EBER-1 stimulates gene expression.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 47 REFERENCES
Oncogenic Role of Epstein-Barr Virus-Encoded RNAs in Burkitt’s Lymphoma Cell Line Akata
TLDR
Transfection of the EBER genes into EBV-negative Akata clones restored the capacity for growth in soft agar, tumorigenicity in SCID mice, resistance to apoptotic inducers, and upregulated expression of bcl-2 oncoprotein.
Epstein-Barr Virus Contributes to the Malignant Phenotype and to Apoptosis Resistance in Burkitt’s Lymphoma Cell Line Akata
TLDR
It is the first report that BL-type EBV infection confers apoptosis resistance even in the absence of expression of LMP1 and BHRF1, both of which are known to have an antiapoptotic function.
Conversion of the lymphoma line “BJAB” by Epstein‐Barr virus into phenotypically altered sublines is accompanied by increased C‐myc mRNA levels
The steady‐state level of c‐myc proto‐oncogene mRNA was investigated in the EBV‐negative human B‐lymphoma line BJAB and 2 sublines that have been converted by EBV into stable EBV‐genome‐carrying and
Post‐transcriptional mechanisms of deregulation of MYC following conversion of a human B cell line by Epstein‐Barr virus.
By utilizing an Epstein‐Barr virus (EBV)‐negative Burkitt's lymphoma line (BJAB) and several EBV‐positive sub‐lines derived from it by in vitro infection, it has been shown previously that the
Epstein-Barr Virus Regulates c-MYC, Apoptosis, and Tumorigenicity in Burkitt Lymphoma
TLDR
It is demonstrated that reestablishment of type I latency in EBV-negative Akata cells restores tumorigenicity and that tumorigenic potential correlates with an increased resistance to apoptosis under growth-limiting conditions and is suggested a novel model whereby a restricted latency program of EBV promotes B-cell survival, and thus virus persistence within an immune host, by selectively targeting the expression of c-MYC.
Epstein-Barr Virus Promotes Epithelial Cell Growth in the Absence of EBNA2 and LMP1 Expression
TLDR
It is indicated that EBV infection causes a transformed phenotype on PGE in the setting of possible unregulated cell cycling and renders even established gastric carcinoma cells more malignant via a limited spectrum of viral latent-gene expression.
EAP, a highly conserved cellular protein associated with Epstein‐Barr virus small RNAs (EBERs).
TLDR
It is discovered that the EBERs also associate with a second highly abundant host‐encoded protein designated EAP (EBER associated protein), which contains a potential nuclear localization signal and a highly acidic carboxy terminus, but does not display marked similarity to any other RNA binding proteins.
Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.
Primate cells harboring the Epstein-Barr virus (EBV) genome synthesize large amounts of two small RNAs:EBER 1 and EBER 2 (EBV-encoded RNA). These RNAs are approximately 180 nucleotides long, possess
Homology between Epstein-Barr Virus DNA and Viral DNA from Burkitt's Lymphoma and Nasopharyngeal Carcinoma determined by DNA-DNA Reassociation Kinetics
TLDR
The average number of EBV genomes associated with virus nonproductive cells was estimated as forty to one hundred per cell by a nucleic acid hybridization technique with EBV-specific complementary RNA (cRNA) and cellular DNA, whereas two to five genomes per cell were found by DNA–DNA hybridization on nitrocellulose filters1,2.
Recombinant Epstein-Barr virus with small RNA (EBER) genes deleted transforms lymphocytes and replicates in vitro.
TLDR
The EBER-deleted EBV recombinants should be useful in further evaluating the role of EBERs in EBV infection, and should be applicable to the construction of other EBVs recombinant within 40 kilobases of the EBNA-2 gene.
...
1
2
3
4
5
...