Malignant cell-specific gelatinase activity in human endometrial carcinoma.

  title={Malignant cell-specific gelatinase activity in human endometrial carcinoma.},
  author={Masashi Takemura and Chihiro Azuma and Tadashi Kimura and Yoshihiro Tokugawa and Masaharu Miki and Mayumi Ono and Fumitaka Saji and Osamu Tanizawa},
  volume={70 1},
BACKGROUND The protease activity leading to degradation of the extracellular matrix was compared between human endometrial cancer and normal uterine endometrium. METHODS Conditioned medium from tumor cells and normal endometrial cells was subjected to electrophoresis on sodium dodecyl sulfate (SDS)-polyacrylamide gel containing gelatin as a substrate. After electrophoresis, the gel was stained with Coomassie blue, and then the enzyme activity, expressed as the zone of dye clearing, was… CONTINUE READING