Maleimide as an inhibitor in measurement of erythrocyte glucose-6-phosphate dehydrogenase activity.

@article{Deutsch1978MaleimideAA,
  title={Maleimide as an inhibitor in measurement of erythrocyte glucose-6-phosphate dehydrogenase activity.},
  author={Joseph Deutsch},
  journal={Clinical chemistry},
  year={1978},
  volume={24 6},
  pages={885-9}
}
Erythrocyte glucose-6-phosphate dehydrogenase activity is measured with a centrifugal analyzer by use of a commercial reagent kit and of the reaction glucose-6-phosphate + NADP+ leads to 6-phosphogluconolactone + NADPH. Rate of production of NADPH is measured and related to hemoglobin concentration. Maleimide is added to inhibit further production of NADPH in a secondary reaction by endogenous 6-phosphogluconate dehydrogenase. The method is compared with others that are designed to circumvent… CONTINUE READING