Male sterile mutant from somatic cell culture of rice

  title={Male sterile mutant from somatic cell culture of rice},
  author={Dinghou Ling and Z. R. Ma and W. Y. Chen and Maria F Chen},
  journal={Theoretical and Applied Genetics},
SummaryUsing MS medium supplemented with 6% sucrose and hormones, plantlets were regenerated from the expiants of mature seeds and young panicles of IRs and IR54. Out of 157 regenerated plants (R1), three were found to be male sterile (ms): one from IRs and two from IR54, including a fertile and sterile chimaera. In the second generations (R2) of IR24 and IR54, one line from each segregated into male sterile and fertile plants. These ms plants could be divided into two types with pollen failure… 
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Inheritance of male sterility mutations induced in haploid sorghum tissue culture
A high frequency of male sterile mutants regeneration was shown in callus cultures derived from leaves and panicles of haploid sorghum and a spontaneous autodiploid obtained from this haploid.
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A restorable variation for a cytoplasmic type of male sterile line is revealed by in vitro culture of rice male sterile lines IR66707A and IR69700A, which could be used in hybrid rice production.


A case of cytoplasmic male sterility controlled by a restoration gene Rf was found in cultivated rice, Oryza sativa L. sativa, and the restoration gene was found to be of gametophytic type.
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Plants carrying Texas male-sterile (Tms) cytoplasm are normally sensitive to Drechslera maydis T-toxin but the progeny of the regenerants showed the phenotype of the female parent with respect to pollen-fertility, and T- toxin resistance.
Mitochondrial DNA variation in maize plants regenerated during tissue culture selection
The mtDNA analyses indicated that the male-fertile, toxin-resistant lines did not originate from selection of N mitochondrial genomes coexisting previously with T genomes in the T cytoplasm line used for selection.
Somaclonal genetics of rice, Oryza sativa L.
The inheritance and variations of some traits of more than 2,000 somatic cell derived plants of rice (Oryza sativa L.) were investigated in the second and third generations (T2 and T3) of regenerated plants (somaclones), and variations phenotyped in T2 proved to breed true.
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The majority of plants recovered from anther calli of the cultivar ‘Calrose 76’ were similar to the starting cultivar but plants regenerated from microspore calli had a wider range of responses than controls for several characteristics.
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Efficient tissue culture and regeneration methods were established using immature wheat embryos as expiants and extensive somaclonal variation for morphological and biochemical traits was observed among 142 regenerants of a Mexican breeding line, ‘Yaqui 50E' and their progeny.
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This is the first report about a disease resistant mutation obtained successfully in rice by tissue culture and in vitro screening with phytotoxin.
Direct development of plantlets from immature panicles of rice in vitro
It is suggested that spikelet budding occurs by the reversion of floral primordia to vegetative stage or by the formation of adventitious buds from epidermal cells.
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It is argued that this variation in plant cell culture itself generates genetic variability (somaclonal variation) that may be employed to enhance the exchange required in sexual hybrids for the introgression of desirable alien genes into a crop species.
The acetocarmine smear technic.
An effort was made to bring together into one paper the work-a-day details to be followed in collecting specimens and making preparations of plant chromosomes at prophase, of condensed chromosomes at meiosis and mitosis, and of coiled chromonemata.