Male-specific DNA markers from African catfish (Clarias gariepinus)

  title={Male-specific DNA markers from African catfish (Clarias gariepinus)},
  author={Bal{\'a}zs Kov{\'a}cs and S{\'a}ndor Egedi and Rich{\'a}rd B{\'a}rtfai and Laszlo Orban},
We searched for sex-specific DNA sequences in the male and female genomes of African catfish, Clarias gariepinus (Burchell, 1822) by comparative random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Two sex-linked RAPD markers were identified from the male DNA pool and confirmed on individual samples, showing good agreement with phenotypic sex. Both markers were isolated, cloned and characterized. The first marker (CgaY1) was nearly 2.6 kb long, while the length of… CONTINUE READING


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