Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation

@article{Eggan2002MaleAF,
  title={Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation},
  author={Kevin C. Eggan and Anja Rode and Isabell Jentsch and Caroline Samuel and Thomas Hennek and Hartmut Tintrup and Branko Zevnik and Jennifer A Erwin and Janet Loring and Laurie Jackson-Grusby and Michael R. Speicher and Ralf K{\"u}hn and Rudolf Jaenisch},
  journal={Nature Biotechnology},
  year={2002},
  volume={20},
  pages={455-459}
}
We have devised a general strategy for producing female mice from 39,X0 embryonic stem (ES) cells derived from male cell lines carrying a targeted mutation of interest. We show that the Y chromosome is lost in 2% of subclones from 40,XY ES cell lines, making the identification of targeted 39,X0 subclones a routine procedure. After gene targeting, male and female mice carrying the mutation can be generated by tetraploid embryo complementation from the 40,XY ES cell line and its 39,X0 derivatives… CONTINUE READING