Maize chlorotic mottle machlomovirus expresses its coat protein from a 1.47-kb subgenomic RNA and makes a 0.34-kb subgenomic RNA.

@article{Scheets2000MaizeCM,
  title={Maize chlorotic mottle machlomovirus expresses its coat protein from a 1.47-kb subgenomic RNA and makes a 0.34-kb subgenomic RNA.},
  author={Kay Scheets},
  journal={Virology},
  year={2000},
  volume={267 1},
  pages={
          90-101
        }
}
  • K. Scheets
  • Published 1 February 2000
  • Biology
  • Virology
Analysis of double-stranded RNAs produced in maize plants infected with maize chlorotic mottle machlomovirus (MCMV) and Northern blots of total RNA from infected plants or protoplasts showed two subgenomic RNAs (sgRNAs). Primer extension was used to map these sgRNAs, which are 1.47 and 0.34 kb long. The transcription start sites are nucleotide (nt) 2970 or 2971 for sgRNA1 and nt 4101 for sgRNA2. The 5' ends of the sgRNAs are similar to one another and to the 5' end of genomic RNA, and 11 nt… 
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