Maize (Zea mays) Hi-II Transformation via Agrobacterium-Mediated T-DNA Transfer.

@article{Lee2016MaizeM,
  title={Maize (Zea mays) Hi-II Transformation via Agrobacterium-Mediated T-DNA Transfer.},
  author={Hyeyoung Lee and Zhanyuan J. Zhang},
  journal={Current protocols in plant biology},
  year={2016},
  volume={1 1},
  pages={
          121-137
        }
}
Genetic transformation of maize via Agrobacterium tumefaciens is still more art than science, with different researchers achieving substantially different transformation results. This article describes our advanced Agrobacterium-mediated transformation system in Hi-II maize. The system utilizes simple binary vectors and immature embryos for the transformation, employing the bar gene as a plant selectable marker in combination with bialaphos for subsequent culture selection. The transformation… 
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