Maintenance of testosterone production by purified adult rat leydig cells for 3 days in vitro

@article{Klnefelter1989MaintenanceOT,
  title={Maintenance of testosterone production by purified adult rat leydig cells for 3 days in vitro},
  author={Gary R. Klnefelter and Larry Larue Ewing},
  journal={In Vitro Cellular & Developmental Biology},
  year={1989},
  volume={25},
  pages={283-288}
}
Using a preparation of highly purified, adult rat Leydig cells and conditions of culture which we found to optimize testosterone production during 24 h, we sought to maintain optimal testosterone production for 3 d. Leydig cells cultured on Cytodex 3 beads at 19% O2 in Dulbecco's modified Eagle's medium-Ham's nutrient mixture F12 (1:1; vol/vol) containing 0.5 mg/ml, total bovine lipoproteins (<1.222 g/ml) with maximal luteinizing hormone (LH) stimulation failed to maintain a constant amount of… CONTINUE READING

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