Maintenance of differentiated rat hepatocytes in primary culture.

@article{Isom1985MaintenanceOD,
  title={Maintenance of differentiated rat hepatocytes in primary culture.},
  author={Harriet C. Isom and Timothy Secott and Ingo Georgoff and Craig Woodworth and J G Mummaw},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1985},
  volume={82 10},
  pages={3252-6}
}
Normal adult rat hepatocytes remained viable and functional for at least 43 days when plated on collagen-coated dishes and fed chemically defined medium supplemented with dimethyl sulfoxide (Me2SO). Hepatocytes isolated by collagenase perfusion and cultured in the presence or absence of Me2SO were (i) examined by light and electron microscopy for… CONTINUE READING