Maintenance of Intracellular pH and Acid Tolerance in Rhizobium meliloti

@article{OHara1989MaintenanceOI,
  title={Maintenance of Intracellular pH and Acid Tolerance in Rhizobium meliloti},
  author={Graham O’Hara and Thomas J. Goss and Michael J. Dilworth and Andrew R. Glenn},
  journal={Applied and Environmental Microbiology},
  year={1989},
  volume={55},
  pages={1870 - 1876}
}
The development and function of the Rhizobium meliloti-Medicago sp. symbiosis are sensitive to soil acidity. Physiological criteria that can be measured in culture which serve to predict acid tolerance in soil would be valuable. The intracellular pH of R. meliloti was measured using either radioactively labeled weak acids (5,5-dimethyloxazolidine-2,4-dione and butyric acid) or pH-sensitive fluorescent compounds; both methods gave similar values. Six acid-tolerant strains (WSM419, WSM533, WSM539… Expand
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TLDR
Results suggest that R. meliloti strains suitablemore for inoculation of alfalfa in acid soils may be selected not by simple saprophytic properties but by their stimulation by the host legume in acid soil. Expand
PERFORMANCE OF Rhizobium meliloti STRAINS SELECTED FOR LOW-pH TOLERANCE
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There were no significant differences in yield and nitrogen fixation within low-pH-tolerant strains of Rhizobium meliloti, and soil pH, inoculum level and cultivar had significant effects on most of the characteristics measured. Expand
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The effects of soil acidity on nitrogen fixation by alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) were investigated in field experiments at 28 locations, and in greenhouseExpand
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