Maintenance of ΔpH by a butanol-tolerant mutant of Clostridium beijerinckii

@article{Wang2005MaintenanceO,
  title={Maintenance of $\Delta$pH by a butanol-tolerant mutant of Clostridium beijerinckii},
  author={Fanqiang Wang and Shelby Kashket and Eva R. Kashket},
  journal={Microbiology},
  year={2005},
  volume={151},
  pages={607-613}
}
The isolation of Clostridium beijerinckii mutants that are more tolerant of butanol than the wild-type offered the opportunity to investigate whether the membrane activities which are required for maintaining the transmembrane ΔpH (the difference in pH between the cellular interior and exterior) are sensitive targets of butanol toxicity. The ΔpH was measured by the accumulation of [14C]benzoate using late-exponential-phase cells which were suspended in citrate/phosphate buffer at pH 5 (to… 

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