Maintenance and killing efficiency of conditional lethal constructs inPseudomonas putida

Abstract

Conditional lethal (suicidal) genetic constructs were designed and employed in strains of Pseudomonads as models for containment of geneticallyengineerd microbes that may be deliberately released into the environment. A strain ofPseudomonas putida was formed with a suicide vector designated pBAP24h that was constructed by cloning the host killing gene (hok… (More)
DOI: 10.1007/BF01583839

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