Urease from cotton (Gossypium hirsutum) seeds: isolation, physicochemical characterization, and antifungal properties of the protein.
Novel sources of urease from leguminosae were selected for activity test, one the them (Prosopis farcta) showed high specific activity (36.52 U mg). The active uerase extract then subjected to series of purification steps involved salting out, DEAE, CM ion exchange and size exclusion chromatography. Specific activity were 52.44, 193.27, 230.55, 433.33 U mg respectively. Main features of urease were detected included estimation of molecular weight (95.500 kDa), optimum pH for activity (8) and stability (7-8), optimum temperature for activity (45C) and stability (25-30 C).