Main properties of urease partially purified from seeds of Syrian mesquite (Prosopis farcta)

Abstract

Novel sources of urease from leguminosae were selected for activity test, one the them (Prosopis farcta) showed high specific activity (36.52 U mg). The active uerase extract then subjected to series of purification steps involved salting out, DEAE, CM ion exchange and size exclusion chromatography. Specific activity were 52.44, 193.27, 230.55, 433.33 U mg respectively. Main features of urease were detected included estimation of molecular weight (95.500 kDa), optimum pH for activity (8) and stability (7-8), optimum temperature for activity (45C) and stability (25-30 C).

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Cite this paper

@inproceedings{Hamzah2014MainPO, title={Main properties of urease partially purified from seeds of Syrian mesquite (Prosopis farcta)}, author={Nazar Abdulameer Hamzah}, year={2014} }