Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo

  title={Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo},
  author={Franz Scherer and Martina Anton and Ulrike Schillinger and Julia Henke and Christian Bergemann and Achim Kr{\"u}ger and Bernd Gänsbacher and Christian Plank},
  journal={Gene Therapy},
Low efficiencies of nonviral gene vectors, the receptor-dependent host tropism of adenoviral or low titers of retroviral vectors limit their utility in gene therapy. To overcome these deficiencies, we associated gene vectors with superparamagnetic nanoparticles and targeted gene delivery by application of a magnetic field. This potentiated the efficacy of any vector up to several hundred-fold, allowed reduction of the duration of gene delivery to minutes, extended the host tropism of adenoviral… 

Magnetofection: Enhancing and Targeting Gene Delivery with Superparamagnetic Nanoparticles and Magnetic Fields

The attractive forces of magnetic gradient fields on superparamagnetic nanoparticles are exploited to potentiate the efficacies, improve the kinetics and dose response, and hence the efficacy of the gene transfer process is improved.

Magnetic Nanoparticles Enhance Adenovirus Transduction In Vitro and In Vivo

It is validated that magnetic cell separation and adenoviral transduction can be accomplished in one reliable integrated and safe system.

Magnetic field-assisted gene delivery: achievements and therapeutic potential.

The availability of stable, nontoxic MNP-gene vector complexes now offers the opportunity to develop magnetic gene targeting (MGT), a variant of MDT in which the gene coding for a therapeutic molecule, rather than the molecule itself, is delivered to a therapeutic target area in the body.

Magnetic targeting strategies in gene delivery.

The latest progress in the development of different magnetic vectors, based on both viral and nonviral gene delivery agents, and a description of magnetic targeting applications in stem cells and in vivo, which has gained interest in recent years due to the rapid development of technology.

Pleiotropic functions of magnetic nanoparticles for ex vivo gene transfer.

Rapidly Transducing and Spatially Localized Magnetofection Using Peptide-Mediated Non-Viral Gene Delivery Based on Iron Oxide Nanoparticles

High cell membrane targeting by GET-DNA and MNP co-complexes and magnetic fields allowed further enhancement to endocytotic uptake, meaning that the nucleic acid cargo was rapidly internalized beyond that of GET complexes alone (GET-DNA).

Nucleic acid delivery using magnetic nanoparticles: the Magnetofection technology.

The principles of the Magnetofection technology and its benefits as compared with standard transfection methods are discussed and future trends in the development of new magnetic nanoparticle formulations will be outlined.

Magnetic concentration of a retroviral vector using magnetite cationic liposomes.

The results suggest that this technique provides a promising approach to capturing and concentrating viral vectors, thus achieving high transduction efficiency and the ability to deliver genes to a specific injured site by applying a magnetic field.

Magnetic Manipulation of a Retroviral Vector Using Magnetite Cationic Liposomes

The results suggest that this new technique provides a promising approach to capture and concentrate viral vectors, thus achieving high transduction efficiency and the ability to deliver genes to a specifically injured site by applying a magnetic field.

Advances in Gene Delivery Systems

The rationale for the design of viral, nonviral and physical methods for gene delivery, as well as advantages and disadvantages of each of the most commonly used gene delivery methods, are explained and future perspectives are provided.



Protective copolymers for nonviral gene vectors: synthesis, vector characterization and application in gene delivery

Novel copolymers of poly(ethylene glycol) and reactive linkers which are derivatized with anionic peptides after copolymerization are designed and applied to coat positively charged nonviral gene vectors by electrostatic interactions.

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.

Intratracheal gene delivery to the mouse airway: characterization of plasmid DNA expression and pharmacokinetics.

'naked' plasmid DNA should be included as a control in all studies on intratracheal gene delivery using nonviral systems, suggesting that transfection is not dependent on damage to airway cells caused by a hypo-osmotic delivery vehicle.

Enhancement of transfection by physical concentration of DNA at the cell surface

It is predicted that manipulations aimed at optimizing DNA complexation or membrane fusion have a fundamental physical limit; new methods designed to increase transfection efficiency must increase DNA concentration at the target cell surface without adding to the toxicity.

Streptavidin paramagnetic particles provide a choice of three affinity-based capture and magnetic concentration strategies for retroviral vectors.

Three strategies have been designed to concentrate infectious retroviral vectors from the supernatants of human- and murine- based packaging cells, and magnetic field-dependent localization such as this may enable the in vivo administration of formulations that concentrate Retroviral infection to the required target tissues and organs.

Efficient Gene Transfer into Human CD34+ Cells by a Retargeted Adenovirus Vector

A chimeric vector was generated which contained the short-shafted Ad35 fiber incorporated into an Ad5 capsid and allowed for gene transfer into a broader spectrum of CD34+ cells, including subsets with potential stem cell capacity.

Adenoviral-mediated gene transfer in lymphocytes.

It is shown that the ectopic expression of CAR in various lymphocyte cell lines, which are almost completely resistant to adenovirus infection, is sufficient to facilitate the efficient transduction of these cells by recombinantadenoviruses.

Variability of adenovirus receptor density influences gene transfer efficiency and therapeutic response in head and neck cancer.

The identification of a 5-fold variation in adenovirus receptor density among tumor cell populations, even of the same histology, greatly influences transduction efficiency and therapeutic results of a variety of adanovirus-based gene therapy strategies supports consideration of assaying adenvirus receptor status, even in tumors of thesame histology from patients enrolled in gene therapy clinical trials.

Tissue-specific targeting of retroviral vectors through ligand-receptor interactions.

A chimeric protein containing the polypeptide hormone erythropoietin and part of the env protein of ecotropic Moloney murine leukemia virus was engineered into the virus, and this murine virus became several times more infectious for murine cells bearing the erythroietin receptor.