Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo

@article{Scherer2002MagnetofectionEA,
  title={Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo},
  author={Franz Scherer and Martina Anton and Ulrike Schillinger and Julia Henke and Christian Bergemann and Achim Kr{\"u}ger and Bernd Gänsbacher and Christian Plank},
  journal={Gene Therapy},
  year={2002},
  volume={9},
  pages={102-109}
}
Low efficiencies of nonviral gene vectors, the receptor-dependent host tropism of adenoviral or low titers of retroviral vectors limit their utility in gene therapy. To overcome these deficiencies, we associated gene vectors with superparamagnetic nanoparticles and targeted gene delivery by application of a magnetic field. This potentiated the efficacy of any vector up to several hundred-fold, allowed reduction of the duration of gene delivery to minutes, extended the host tropism of adenoviral… 

Magnetofection: Enhancing and Targeting Gene Delivery with Superparamagnetic Nanoparticles and Magnetic Fields

TLDR
The attractive forces of magnetic gradient fields on superparamagnetic nanoparticles are exploited to potentiate the efficacies, improve the kinetics and dose response, and hence the efficacy of the gene transfer process is improved.

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It is validated that magnetic cell separation and adenoviral transduction can be accomplished in one reliable integrated and safe system.

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The availability of stable, nontoxic MNP-gene vector complexes now offers the opportunity to develop magnetic gene targeting (MGT), a variant of MDT in which the gene coding for a therapeutic molecule, rather than the molecule itself, is delivered to a therapeutic target area in the body.

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The latest progress in the development of different magnetic vectors, based on both viral and nonviral gene delivery agents, and a description of magnetic targeting applications in stem cells and in vivo, which has gained interest in recent years due to the rapid development of technology.

Pleiotropic functions of magnetic nanoparticles for ex vivo gene transfer.

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High cell membrane targeting by GET-DNA and MNP co-complexes and magnetic fields allowed further enhancement to endocytotic uptake, meaning that the nucleic acid cargo was rapidly internalized beyond that of GET complexes alone (GET-DNA).

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TLDR
The principles of the Magnetofection technology and its benefits as compared with standard transfection methods are discussed and future trends in the development of new magnetic nanoparticle formulations will be outlined.

Magnetic concentration of a retroviral vector using magnetite cationic liposomes.

TLDR
The results suggest that this technique provides a promising approach to capturing and concentrating viral vectors, thus achieving high transduction efficiency and the ability to deliver genes to a specific injured site by applying a magnetic field.

Magnetic Manipulation of a Retroviral Vector Using Magnetite Cationic Liposomes

TLDR
The results suggest that this new technique provides a promising approach to capture and concentrate viral vectors, thus achieving high transduction efficiency and the ability to deliver genes to a specifically injured site by applying a magnetic field.

Advances in Gene Delivery Systems

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The rationale for the design of viral, nonviral and physical methods for gene delivery, as well as advantages and disadvantages of each of the most commonly used gene delivery methods, are explained and future perspectives are provided.
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