Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells
@article{Bulte2001MagnetodendrimersAE, title={Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells}, author={Jeff W. M. Bulte and Trevor Douglas and Brian P. Witwer and Su-Chun Zhang and Erica Strable and Bobbi K. Lewis and Holly A. Zywicke and Bradley R Miller and Peter van Gelderen and Bruce M. Moskowitz and Ian D. Duncan and Joseph A. Frank}, journal={Nature Biotechnology}, year={2001}, volume={19}, pages={1141-1147} }
Magnetic resonance (MR) tracking of magnetically labeled stem and progenitor cells is an emerging technology, leading to an urgent need for magnetic probes that can make cells highly magnetic during their normal expansion in culture. We have developed magnetodendrimers as a versatile class of magnetic tags that can efficiently label mammalian cells, including human neural stem cells (NSCs) and mesenchymal stem cells (MSCs), through a nonspecific membrane adsorption process with subsequent…
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References
SHOWING 1-10 OF 47 REFERENCES
Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells
- BiologyNature Biotechnology
- 2000
A cell labeling approach using short HIV-Tat peptides to derivatize superparamagnetic nanoparticles is developed, which efficiently internalized into hematopoietic and neural progenitor cells in quantities up to 10–30 pg of super paramagnetic iron per cell.
Neurotransplantation of magnetically labeled oligodendrocyte progenitors: magnetic resonance tracking of cell migration and myelination.
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 1999
The present results demonstrate that magnetic resonance tracking of transplanted oligodendrocyte progenitors is feasible and has the potential to be easily extended to other neurotransplantation studies involving different precursor cell types.
High-efficiency intracellular magnetic labeling with novel superparamagnetic-Tat peptide conjugates.
- Biology, ChemistryBioconjugate chemistry
- 1999
A biocompatible, dextran coated superparamagnetic iron oxide particle was derivatized with a peptide sequence from the HIV-tat protein to improve intracellular magnetic labeling of different target cells, and internalized into lymphocytes over 100-fold more efficiently than nonmodified particles.
Scientific and clinical applications of magnetic carriers
- Biology, Chemistry
- 1997
Preparation and Modification of Biodegradable Magnetic Particles: Preparation and Application of Monosized magnetic Particles in Selective Cell Separation and Applications in Molecular Biology and Drug Delivery and Radionuclide Therapy.
Normal T-cell response and in vivo magnetic resonance imaging of T cells loaded with HIV transactivator-peptide-derived superparamagnetic nanoparticles.
- Biology, ChemistryJournal of immunological methods
- 2001
Generation of oligodendroglial progenitors from neural stem cells
- BiologyJournal of neurocytology
- 1998
The progression of stem cells to oligodendroglial progenitors is likely induced by factors in B104CM, and characterization of their expression of early markers, cell cycle, migration, and self-renewal suggests that they were pre-O2A progenitor.
Molecular and Cellular Magnetic Resonance Contrast Agents
- Biology, Chemistry
- 2001
This chapter describes the latest advances in chemical engineering and molecular/cellularbiology that are producing an entirely new class of (targeted) MR contrast agents that can be used for high-resolution imaging of biologic processes at the molecular and cellular level.
Monocrystalline iron oxide nanocompounds (MION): Physicochemical properties
- ChemistryMagnetic resonance in medicine
- 1993
The physicochemical properties of MION are reported using a variety of analytical techniques to show that a novel monocrystalline iron oxide nanocompound, a stable colloid that enables target specific MR imaging, has an inverse spinel crystal structure.
Magnetic Nanoparticles as Contrast Agents for MR Imaging
- Biology
- 1997
This chapter presents an overview of the use of magnetic nanoparticles as contrast agents for MRI, and covers the basic principles of preparation and characterization, including variable-field relaxometry.
Dysprosium-DOTA-PAMAM dendrimers as macromolecular T2 contrast agents. Preparation and relaxometry.
- Chemistry, BiologyInvestigative radiology
- 1998
The large temperature dependence suggests that the dominant mechanism of relaxation is the contact interaction effect, with the proton residence time as the primary time constant, and this largely unexplored relaxation mechanism has the potential to create a new class of T2-selective contrast agents.