Magnetizable solid-phase fluoroimmunoassay of phenytoin in disposable test tubes.

@article{Kamel1980MagnetizableSF,
  title={Magnetizable solid-phase fluoroimmunoassay of phenytoin in disposable test tubes.},
  author={R. S. Kamel and Jonathan Charles Landon and David Shane Smith},
  journal={Clinical chemistry},
  year={1980},
  volume={26 9},
  pages={
          1281-4
        }
}
We developed a fluoroimmunoassay for phenytoin in serum or plasma, based on the magnetic separation technique. The method involves sheep anti-phenytoin serum coupled to magnetizable cellulose/iron oxide particles, with a fluorescein-labeled phenytoin analog as tracer. After magnetic sedimentation of the solid phase from assay mixtures, the free fraction of the tracer is aspirated, removing endogenous fluorophores and other interfering components of the sample. The antibody-bound tracer is then… CONTINUE READING

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