Magnetic selection of transiently transfected cells.

Abstract

An efficient and rapid method for selecting transiently transfected cells is described. A plasmid encoding for a neural cell-specific surface marker is co-transfected into mammalian cells along with the gene of interest. After uptake and expression of these two plasmids, the transfected cells are immuno-adsorbed to magnetic beads pre-coated with antibodies… (More)

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