Corpus ID: 92793835

Magnetic resonance of biomolecules : an introduction to the theory and practice of NMR and ESR in biological systems

  title={Magnetic resonance of biomolecules : an introduction to the theory and practice of NMR and ESR in biological systems},
  author={P. Knowles and D. Marsh and H. Rattle},
Influence of water content and temperature on molecular mobility and intracellular glasses in seeds and pollen
At low temperatures, when storage longevity cannot be determined on a realistic time scale, 2Azz measurements can provide an estimate of the optimum storage conditions and suggest that storage stability might be at least partially controlled by molecular mobility. Expand
Dehydration-induced redistribution of amphiphilic molecules between cytoplasm and lipids is associated with desiccation tolerance in seeds.
In desiccation-intolerant radicles, partitioning of spin probes into lipids during dehydration occurred at higher water contents compared with tolerant and PEG-induced tolerant radicle, suggesting that the microviscosity of the cytoplasm may control the partitioning behavior in dehydrating seeds. Expand
High critical temperature above T(g) may contribute to the stability of biological systems.
Differences in temperature-dependent mobilities above T(g) suggest that proteins rather than sugars play an important role in the intracellular glass formation, which is expected to provide excellent long-term stability to dry organisms, maintaining a slow molecular motion in the cytoplasm even at temperatures far above T (g). Expand
Measuring plasma-generated ·OH and O atoms in liquid using EPR spectroscopy and the non-selectivity of the HTA assay
Plasma-generated hydroxyl radicals (·OH) and oxygen atoms (O) produced by the COST reference plasma jet, a micro-scaled atmospheric pressure plasma jet, were investigated using a variety ofExpand
Electron spin resonance studies on deuterated nitroxyl spin probes used in Overhauser‐enhanced magnetic resonance imaging
The deuterated nitroxyl radicals are suitable spin probes for in vivo/in vitro electron spin resonance and Overhauser‐enhanced magnetic resonance imaging modalities. Expand
Structural insights on biologically relevant cationic membranes by ESR spectroscopy
This review is the extensive structural characterization of cationic membranes prepared with dioctadecyldimethylammonium bromide or diC14-amidine to illustrate how ESR spectroscopy can provide important structural information on bilayer thermotropic behavior, gel and fluid phases, phase coexistence, presence of bilayer interdigitation, membrane fusion and interactions with other biologically relevant molecules. Expand
Comparative Study of the Reduction Process of Different Ring Structured Nitroxyl Spin Probes: An Electron Spin Resonance Study
Electron spin resonance spectroscopy (ESR) studies on the reduction process of nitroxyl spin probes were carried out for 1mM concentration of 14N-labeled pyrrolidine nitroxyl spin probes,Expand
ESR line width and line shape dependence of Overhauser‐enhanced magnetic resonance imaging
The agent concentration, line width and Lorentzian component are the important factors in determining the Overhauser‐enhancement factor and the agent concentration was optimized as 2.5–3 mm for in vivo/in vitro electron spin resonance imaging and Overhausers‐enhanced magnetic resonance imaging phantom studies. Expand
A decrease in bulk water and mannitol and accumulation of trehalose and trehalose-based oligosaccharides define a two-stage maturation process towards extreme stress resistance in ascospores of Neosartorya fischeri (Aspergillus fischeri).
A novel two-step model for the acquisition of extreme stress resistance and entry into dormancy is provided for Neosartorya fischeri and it is indicated that this stage is associated with the removal of bulk water and an increase of chemical stability. Expand