Magnetic enzyme-linked immunosorbent assay (MELISA) for determination of specific IgG in paracoccidioidomycosis.

@article{Camargo1984MagneticEI,
  title={Magnetic enzyme-linked immunosorbent assay (MELISA) for determination of specific IgG in paracoccidioidomycosis.},
  author={Z P de Camargo and Jean-Luc Guesdon and Edouard Drouhet and Luce Improvisi},
  journal={Sabouraudia},
  year={1984},
  volume={22 4},
  pages={
          291-9
        }
}
A magnetic solid phase enzyme-linked immunosorbent assay (MELISA) for quantification of IgG antibodies to somatic and metabolic antigens of Paracoccidioides brasiliensis was developed. Activation of magnetic polyacrylamide agarose beads with concanavalin A was superior to glutaraldehyde activation, and test sensitivity was higher for somatic than for metabolic antigens. Comparative MELISA, counterimmunoelectrophoresis and erythroimmunoassay tests with sera from 33 proven cases of… CONTINUE READING
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