Magnetic carbohydrate nanoparticles for affinity cell separation.


Magnetically responsive nanoparticles were prepared from enzymatically hydrolysed starch and magnetite. Two different monoclonal antibodies were covalently coupled to the particles. The antibody-coupled particles were in the size range of 100-300 nm and had an iron content of about 60%. Using 100 micrograms of magnetic particles (coupled with monoclonal mouse anti-rat Ig kappa light chain antibody) a very high depletion of surface Ig positive cells (mostly B-cells) from one million rat peripheral blood mononuclear cells could be achieved. The separation efficiency was evaluated by flow cytofluorometric analysis. This technique permits the detection of a small number of surface Ig positive cells among 10,000 negative cells.

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@article{Schroeder1986MagneticCN, title={Magnetic carbohydrate nanoparticles for affinity cell separation.}, author={Ulla Schroeder and Sverker Segr{\'e}n and C Gemmefors and Gunnar Hedlund and Bo Jansson and Hans Olov Sj{\"{o}gren and Carl A. K. Borrebaeck}, journal={Journal of immunological methods}, year={1986}, volume={93 1}, pages={45-53} }