Magnetic carbohydrate nanoparticles for affinity cell separation.

Abstract

Magnetically responsive nanoparticles were prepared from enzymatically hydrolysed starch and magnetite. Two different monoclonal antibodies were covalently coupled to the particles. The antibody-coupled particles were in the size range of 100-300 nm and had an iron content of about 60%. Using 100 micrograms of magnetic particles (coupled with monoclonal mouse anti-rat Ig kappa light chain antibody) a very high depletion of surface Ig positive cells (mostly B-cells) from one million rat peripheral blood mononuclear cells could be achieved. The separation efficiency was evaluated by flow cytofluorometric analysis. This technique permits the detection of a small number of surface Ig positive cells among 10,000 negative cells.

Cite this paper

@article{Schroeder1986MagneticCN, title={Magnetic carbohydrate nanoparticles for affinity cell separation.}, author={Ulla Schroeder and Sverker Segr{\'e}n and C Gemmefors and Gunnar Hedlund and Bo Jansson and Hans Olov Sj{\"{o}gren and Carl A. K. Borrebaeck}, journal={Journal of immunological methods}, year={1986}, volume={93 1}, pages={45-53} }