Magnetic capture hybridisation for improved PCR detection ofNectria galligena from lignified apple extracts

@article{Langrell2008MagneticCH,
  title={Magnetic capture hybridisation for improved PCR detection ofNectria galligena from lignified apple extracts},
  author={Stephen R. H. Langrell and Derek J. Barbara},
  journal={Plant Molecular Biology Reporter},
  year={2008},
  volume={19},
  pages={5-11}
}
In order to reduce the effects of inhibitors present in DNA extracts from lignified apple tissues, a magnetic capture-hybridisation PCR (MCH-PCR) technique was developed forNectria galligena using the ITS 1 region of the rRNA gene repeats as target. The trapping reagent used to coat the magnetic beads was an 81 bp single-stranded DNA oligonucleotide biotin-labelled on the 5é-terminal and designed to be complementary to part of the rRNA gene ITS 1 region ofN. galligena. For specificity, the… CONTINUE READING
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