Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA.

@article{Maher2001MagneticBC,
  title={Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA.},
  author={Nancy Maher and H. Kenneth Dillon and Sten H Vermund and Thomas R. Unnasch},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 1},
  pages={449-52}
}
PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.