Magnetic assembly of high-density DNA arrays for genomic analyses.

  title={Magnetic assembly of high-density DNA arrays for genomic analyses.},
  author={Kristopher D. Barbee and Xiaohua Huang},
  journal={Analytical chemistry},
  volume={80 6},
A method for rapidly assembling high-density DNA arrays with near-perfect order is described. Photolithography is used to generate a wafer-scale array of microwells in a layer of photoresist on a chemically functionalized glass coverslip. The array is enclosed within a microfluidic device, and a suspension of superparamagnetic microbeads conjugated to DNA molecules is introduced into the chamber. A permanent magnet is used to direct the rapid assembly of the beads into the wells, with each well… CONTINUE READING