Magnetic albumin/protein A immunomicrospheres. II. Specificity, reproducibility, and resolution of the magnetic cell separation technique.

Abstract

Magnetically responsive albumin/protein A immunomicrospheres (MIMS) were prepared by reacting a mixture of albumin, iron oxide, and protein A in a two-phase emulsion coagulation procedure. The protein A ligand permits strong affinity binding of the monoclonal anti-HLA BW6 antibody to the 500-nm MIMS in a one-step process. HLA BW6+ and BW4+ human peripheral… (More)

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