Magnesium-induced assembly of a complete DNA polymerase catalytic complex.

Abstract

The molecular details of the nucleotidyl transferase reaction have remained speculative, as strategies to trap catalytic intermediates for structure determination utilize substrates lacking the primer terminus 3'-OH and catalytic Mg2+, resulting in an incomplete and distorted active site geometry. Since the geometric arrangement of these essential atoms… (More)
DOI: 10.1016/j.str.2006.01.011

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